Pseudotemporal and spatial organization of B cell activation and differentiation in Peyer's patches

Abstract Organized lymphoid tissues are important sites of immune induction where B cells activate and differentiate. High-resolution transcriptomic technology has revolutionized current understandings cell activation differentiation in organized such as lymph nodes but not been used to dissect Peyer’s patches, intestinal induction. We utilized single-cell RNA sequencing spatial transcriptomics define pseudotemporal organization, respectively, porcine jejunal ileal patches (JPPs IPPs). IPPs had greater follicular mass more defined borders than JPPs, resulting abundance non-follicular JPPs. Despite morphology abundances varying between JPPs IPPs, trajectories predicted locations were similar locations. Transition resting into was demarcated by increased expression follicle- cycle-associated genes movement from interfollicular zones (IFZs) follicles. Another trajectory indicated transition located IFZs directly antibody-secreting crypts, suggesting a route extrafollicular differentiation. Altogether, results indicate conserved patterns both across locations, despite differences cellular morphology. Research funded (1) USDA-ARS CRIS project #5030-3200- 225-00D, (2) the SCINet USDA ARS number 0500-00093-001- 00-D, (3) an appointment Agricultural Service (ARS) Participation Program administered Oak Ridge Institute for Science Education (ORISE) through interagency agreement U.S. Department Energy (DOE) Agriculture (USDA). ORISE is managed ORAU under DOE contract DE-SC0014664. All opinions expressed this paper authors’ do necessarily reflect policies views USDA, ARS, DOE, or ORAU/ORISE.